Protein array to find K-met specific readers. -CANDOR chip has 100 GST-fusion proteins on a nitrocellulose slide. -Tested Human tudor, SANT, MBT, PHD, Chromo, and Bromo proteins. -probe w. Cy3 peptides: R-me2, H3K79dimethyl, and H3K79-unmethyl doesnt show known 53BP1 interaction. -probe w. H3K4met1-3, H3K9-met1-3, H4K20me1-3. Chromodomains bound H3K9 (verifies HP1) Tudor domains bound all three (JMJD2A bound di and ri H3K4 and H4K20).. MBT bound only H3K4 and H4K20. -verified chip w. peptide pull down. Biotinylated peptides on strep beads incubates w. GST fusions. -new protein CDY1 w. chromodomain. found on y chromosome. implicated w. make fertility. -this approach identified MBT at K-met readers.