Michael Lovett (Washington University School of Medicine): Capture biotin-BAC plus hybridized genomic fragments on strptavidin beads and then PCR amplify with specific primers. Result: 52% from the target region

Gregory Hannon (Cold Spring Harbor Laboratory): We have developed a method of using flexible, high-density microarrays to capture more than 200,000 protein-coding exons. All exons found to be shorter than 135 bases were extended in both the 5' and 3' directions to include a minimum of 135 bases of genomic sequence. Overlapping microarray probes (>60 bases) were designed to span each target region, with a probe positioned every 20 bases for the forward strand of the genome. A set of seven arrays (Nimblegen) was created to capture all sequences, with each array containing 385,000 probes. Up to 55–85% of the captured fragments are associated with targeted regions and up to 98% of intended exons can be recovered.