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Rapid specific amplification of rat antibody cDNA from nine hybridomas in the presence of myeloma light chains
Journal of Immunological Methods 315 (1-2), (2006)
info:doi/10.1016/j.jim.2006.07.002
Posted by harijay to light chain Light-Chain monoclonal sequencing on Thu Sep 27 2007 at 20:03 UTC | info | related
 
Efficient amplification and direct sequencing of mouse variable regions from any immunoglobulin gene family
FEBS Letters 452 (3), (1999)
info:doi/10.1016/s0014-5793(99)00649-3
Good paper on link between primer design and Kabat and Wu database
Posted by harijay to light chain Light-Chain monoclonal sequencing on Thu Sep 27 2007 at 18:00 UTC | info | related
 
Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3' to 5' exonuclease activity.
Z Wang et al.
Journal of immunological methods 233 (1-2), (13 Jan 2000)
info:pmid/10648866
This is the paper which gave the primer sequences we used for round II of the monoclonal sequencing experiment
Posted by harijay to Light-Chain light chain monoclonal sequencing on Thu Sep 27 2007 at 17:55 UTC | info | related
 
Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain reaction.
C Kettleborough et al.
European journal of immunology 23 (1), (Jan 1993)
info:pmid/8419173
Posted by harijay to light chain Light-Chain monoclonal sequencing on Thu Sep 27 2007 at 17:50 UTC | info | related
 
Hybridoma fusion cell lines contain an aberrant kappa transcript
Molecular Immunology 25 (10), (1988)
info:doi/10.1016/0161-5890(88)90005-3
Aberrant contaminating kappa mRNA
Posted by harijay to Light-Chain light chain monoclonal sequencing on Thu Sep 27 2007 at 17:48 UTC | info | related
 
By-passing immunization Human antibodies from V-gene libraries displayed on phage
Journal of Molecular Biology 222 (3), 581 (1991)
info:doi/10.1016/0022-2836(91)90498-u
Posted by harijay to light chain Light-Chain monoclonal sequencing rpas on Tue Sep 25 2007 at 19:43 UTC | info | related
 
Making antibody fragments using phage display libraries.
T Clackson et al.
Nature 352 (6336), 624-8 (15 Aug 1991)
info:pmid/1907718 | info:doi/10.1038/352624a0
Posted by harijay to rpas light chain Light-Chain monoclonal sequencing on Tue Sep 25 2007 at 19:38 UTC | info | related
 
Reshaping human antibodies: grafting an antilysozyme activity.
M Verhoeyen, C Milstein, and G Winter
Science (New York, N.Y.) 239 (4847), 1534-6 (25 Mar 1988)
info:pmid/2451287
Posted by harijay to rpas Light-Chain light chain monoclonal sequencing on Tue Sep 25 2007 at 19:37 UTC | info | related
 
Man-made antibodies
Nature 349 (6307), 293 (1991)
info:doi/10.1038/349293a0
Posted by harijay and 1 other to Light-Chain light chain monoclonal sequencing rpas on Tue Sep 25 2007 at 18:58 UTC | info | related
 
VBASE2 - the integrative germ-line V gene database
www.vbase2.org
Posted by harijay and 1 other to monoclonal sequencing Light-Chain light chain on Fri Sep 21 2007 at 16:44 UTC | info | related

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