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Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies
W Yuan et al.
Archives of biochemistry and biophysics 394 (1), 87-98 (01 Oct 2001)
info:pmid/11566031 | info:doi/10.1006/abbi.2001.2522
Posted by gjwilliams2 to Gammaproteobacteria isolation & Acyltransferases Coenzyme A betaproteobacteria Recombinant Fusion Proteins Substrate Specificity isolation & purification Enzymology purification U.S. Gov't P.H.S. Research Support binding sites kinetics metabolism genetics chemistry classification on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Purification, priming, and catalytic acylation of carrier protein domains in the polyketide synthase and nonribosomal peptidyl synthetase modules of the HMWP1 subunit of yersiniabactin synthetase
Z Suo, C C Tseng, and C T Walsh
Proceedings of the National Academy of Sciences of the United States of America 98 (1), 99-104 (02 Jan 2001)
info:pmid/11134531 | info:doi/10.1073/pnas.021537498
Posted by gjwilliams2 to Pantetheine Acyl Carrier Protein Siderophores Thiazoles Peptide Synthases Holoenzymes isolation & Malonyl Coenzyme A Acyltransferases Protein Subunits Bacillus subtilis Multienzyme Complexes Recombinant Proteins Apoproteins analogs & derivatives Acylation Bacterial Proteins Tertiary isolation & purification Peptide Fragments Protein Processing Post-Translational Enzymology Molecular Weight Yersinia pestis purification U.S. Gov't Phenols Non-U.S. Gov't Escherichia coli P.H.S. Research Support Molecular cysteine kinetics metabolism protein structure genetics cloning molecular structure chemistry on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Cloning, sequencing, overproduction and purification of trypanothione reductase from Trypanosoma cruzi
F X Sullivan and C T Walsh
Molecular and biochemical parasitology 44 (1), 145-7 (Jan 1991)
info:pmid/2011150
Posted by gjwilliams2 to isolation & NADPH Oxidoreductases Trypanosoma cruzi Genetic Vectors Recombinant Proteins Base Sequence Enzymology Molecular Sequence Data Amino Acid Sequence purification U.S. Gov't BIOSYNTHESIS Non-U.S. Gov't Escherichia coli P.H.S. Research Support Molecular NADH animals genetics cloning on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Expression, purification, and characterization of SH2-containing protein tyrosine phosphatase, SH-PTP2
S Sugimoto et al.
The Journal of biological chemistry 268 (30), 22771-6 (25 Oct 1993)
info:pmid/8226787
Posted by gjwilliams2 to Platelet-Derived Growth Factor isolation & Protein-Tyrosine-Phosphatase Ion Exchange Recombinant Proteins Phosphopeptides Substrate Specificity Polyacrylamide Gel isolation & purification Peptide Fragments Molecular Weight Epidermal Growth Factor Molecular Sequence Data Amino Acid Sequence Hydrogen-Ion Concentration purification receptors U.S. Gov't Electrophoresis BIOSYNTHESIS Non-U.S. Gov't thermodynamics Escherichia coli COMPARATIVE STUDY humans P.H.S. Research Support Molecular kinetics metabolism Chromatography cloning Receptor on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Mutational analysis of posttranslational heterocycle biosynthesis in the gyrase inhibitor microcin B17: distance dependence from propeptide and tolerance for substitution in a GSCG cyclizable sequence
R Sinha Roy, P J Belshaw, and C T Walsh
Biochemistry 37 (12), 4125-36 (24 Mar 1998)
info:pmid/9521734 | info:doi/10.1021/bi9728250
Posted by gjwilliams2 to Research Support, U.S. Gov't, P.H.S. Research Support, Non-U.S. Gov't DNA Topoisomerases, Type II Protein Processing, Post-Translational Mutagenesis, Site-Directed Bacteriocins isolation & ATP-Binding Cassette Transporters Sequence Deletion DNA Mutational Analysis Escherichia coli Proteins Point Mutation Carrier Proteins Protein Precursors antagonists & inhibitors Monosaccharide Transport Proteins Multienzyme Complexes Amino Acid Substitution Recombinant Fusion Proteins Substrate Specificity Bacterial Proteins Base Sequence Peptides Molecular Sequence Data Amino Acid Sequence purification BIOSYNTHESIS metabolism chemical synthesis genetics on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Vicenistatin, a novel 20-membered macrocyclic lactam antitumor antibiotic
K Shindo et al.
The Journal of antibiotics 46 (7), 1076-81 (Jul 1993)
info:pmid/8360102
Posted by gjwilliams2 to Drug Screening Assays Lactams isolation & macrolides Aminoglycosides antitumor Antineoplastic Anti-Bacterial Agents Magnetic Resonance Spectroscopy Nude Tumor Cells Cultured purification BIOSYNTHESIS humans Pharmacology antibiotics Streptomyces metabolism animals Fermentation mice molecular structure chemistry classification on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Optical processing of bacterial libraries for directed evolution
Allan Wallace Scruggs and Neal W Woodbury
Biotechnology and bioengineering 84 (4), 445-51 (20 Nov 2003)
info:pmid/14574702 | info:doi/10.1002/bit.10794
Posted by gjwilliams2 to Research Support, U.S. Gov't, Non-P.H.S. Photosensitizing Agents Dose-Response Relationship, Radiation Microscopy, Fluorescence PHOTOMETRY isolation & Coculture Techniques Directed Molecular Evolution Cell Separation radiation effects drug effects optics purification Escherichia coli cell division Light Pharmacology flow cytometry methods classification on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Regeneration of misprimed nonribosomal peptide synthetases by type II thioesterases
Dirk Schwarzer et al.
Proceedings of the National Academy of Sciences of the United States of America 99 (22), 14083-8 (29 Oct 2002)
info:pmid/12384573 | info:doi/10.1073/pnas.212382199
Posted by gjwilliams2 to Tyrocidine Acyl Carrier Protein Peptide Synthases Cyclic Bacitracin isolation & Fatty Acid Synthetase Complex Malonyl Coenzyme A Thiolester Hydrolases Enzyme Activation Escherichia coli Proteins Catalysis Ribosomes Recombinant Fusion Proteins Apoproteins Bacterial Proteins isolation & purification Enzymology Peptides Hydrolysis purification amino acids fatty acids BIOSYNTHESIS kinetics metabolism Bacillus genetics chemistry gene expression on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution
J H Lebbink et al.
Biochemistry 39 (13), 3656-65 (04 Apr 2000)
info:pmid/10736164
Posted by gjwilliams2 to isolation & beta-Glucosidase Glucosides Asparagine Directed Molecular Evolution Enzyme Stability Enzyme Activation Catalysis Point Mutation Serine Gene Library Pyrococcus furiosus antagonists & inhibitors Amino Acid Substitution Variation (Genetics) Bacterial Proteins Enzymology Molecular Sequence Data Amino Acid Sequence purification temperature glycosylation mutagenesis Non-U.S. Gov't Research Support binding sites methods heat kinetics metabolism genetics on Thu Jul 03 2008 at 00:15 UTC | info | related
 
The stress response to ionizing radiation involoves c-Abl-dependent phosphorylation of SHPTP1
S Kharbanda et al.
Proceedings of the National Academy of Sciences of the United States of America 93 (14), 6898-6901 (09 Jul 1996)
info:pmid/8692915
Posted by gjwilliams2 to Proto-Oncogene Proteins c-abl Cesium Radioisotopes isolation & radiation effects Intracellular Signaling Peptides and Proteins Ionizing Protein-Tyrosine-Phosphatase 3T3 Cells Ca(2)-Calmodulin Dependent Protein Kinase Recombinant Proteins Protein-Tyrosine Kinase Cell Line Molecular Sequence Data Amino Acid Sequence Tumor Cells transfection Mitogen-Activated Protein Kinases JNK Mitogen-Activated Protein Kinases Cultured purification U.S. Gov't BIOSYNTHESIS DNA damage radiation humans P.H.S. Research Support myeloid binding sites Phosphorylation metabolism leukemia animals mice stress on Thu Jul 03 2008 at 00:15 UTC | info | related

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