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Astrophysical Journal 682, 985-1003 (Aug 2008)
Science 321 (5891), 943-6 (15 Aug 2008)
The EMBO Journal 16 (12), 3373-85 (Jun 1997)
Biochemistry 41 (39), 11731 (2002)
The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound
to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the
recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic
resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a
location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with
a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate
small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the
natural complex described by others, together with the results reported here, suggests potential applications
for small molecule binding by apo-Neocarzinostatin
Structure 6 (4), 429 (1998)
Biochemical and Biophysical Research Communications 184 (2), 1074 (1992)
X-ray crystallographic studies of the two forms of chymosin.
Protein Engineering Design and Selection 11 (10), 833-40 (01 Oct 1998)
1CZI structure for chymosin. Unread
Journal of synchrotron radiation 14 (Pt 1), 51-72 (Jan 2007)
Nature, published online 01 Jul 2007
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