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Biochemical and structural characterization of a novel family of cystathionine beta-synthase domain proteins fused to a Zn ribbon-like domain.
Michael Proudfoot et al.
Journal of molecular biology 375 (1), 301-15 (04 Jan 2008)
info:pmid/18021800 | info:doi/10.1016/j.jmb.2007.10.060
Posted by gabanyi and 1 other to zinc Thermoplasma Amino Acid Sequence Homology Saccharomyces cerevisiae Proteins Rubredoxins Recombinant Proteins Pyrococcus horikoshii isolation & purification Pyrococcus furiosus Tertiary secondary protein structure Protein Conformation Oxidation-Reduction Nad Enzymology myocardium Site-Directed mutagenesis Molecular Sequence Data Molecular Biological Models iron Horses Escherichia coli Dithionite dimerization Cytochromes c cysteine metabolism purification isolation & genetics Cystathionine beta-Synthase Conserved Sequence chemistry analysis Calcium Pharmacology Ascorbic Acid animals Amino Acid Sequence Amino Acid Motifs on Wed Aug 06 2008 at 18:42 UTC | info | related
 
Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide
L You and F H Arnold
Protein engineering 9 (1), 77-83 (Jan 1996)
info:pmid/9053906
Posted by gjwilliams2 to chemistry Subtilisins Non-P.H.S. U.S. Gov't Research Support isolation & purification Recombinant Proteins secondary protein structure Protein engineering polymerase chain reaction Point Mutation Peptides Site-Directed mutagenesis Molecular Sequence Data Models kinetics Gene Library Escherichia coli Enzyme Activation Directed Molecular Evolution dimethylformamide Molecular cloning Base Sequence genetics Enzymology Bacillus subtilis metabolism Anilides on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Structure-based enhancement of the first anomeric glucokinase
Jie Yang, Lesley Liu, and Jon S Thorson
Chembiochem : a European journal of chemical biology 5 (7), 992-6 (05 Jul 2004)
info:pmid/15239058 | info:doi/10.1002/cbic.200400041
Posted by gjwilliams2 to P.H.S. U.S. Gov't Research Support isolation & purification Recombinant Proteins mutation Site-Directed mutagenesis Molecular Sequence Data Molecular Models chemical synthesis analogs & derivatives glucose metabolism genetics chemistry Glucokinase Enzymology Escherichia Carbohydrate Sequence on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli
N Wymer et al.
Structure (London, England : 1993) 9 (1), 1-9 (10 Jan 2001)
info:pmid/11342129
Posted by gjwilliams2 to Substrate Specificity Matrix-Assisted Laser Desorption-Ionization mass spectrometry P.H.S. U.S. Gov't Non-U.S. Gov't Research Support secondary protein structure mutation Site-Directed mutagenesis Molecular chemical Models Lysine kinetics Gene Library Enzymology Escherichia coli X-ray crystallography Catalytic Domain Catalysis binding sites metabolism chemistry Aldehyde-Lyases on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck
D G Winkler et al.
Proceedings of the National Academy of Sciences of the United States of America 90 (11), 5176-80 (01 Jun 1993)
info:pmid/8506364
Posted by gjwilliams2 to Threonine Pharmacology Tetradecanoylphorbol Acetate Serine P.H.S. U.S. Gov't Non-U.S. Gov't Research Support Recombinant Proteins Recombinant Fusion Proteins Protein-Tyrosine Kinase Phosphoserine Phosphorylation Phosphopeptides chemical synthesis Peptides Peptide Mapping Site-Directed mutagenesis Molecular Weight Molecular Sequence Data Lymphocyte Specific Protein Tyrosine Kinase p56(lck) humans Hela Cells metabolism isolation & purification genetics Glutathione Transferase Polyacrylamide Gel Two-Dimensional gel Electrophoresis Cell Line Amino Acid Sequence on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Biosynthetic thiolase from Zoogloea ramigera. Mutagenesis of the putative active-site base Cys-378 to Ser-378 changes the partitioning of the acetyl S-enzyme intermediate
S F Williams et al.
The Journal of biological chemistry 267 (23), 16041-3 (15 Aug 1992)
info:pmid/1353760
Posted by gjwilliams2 to Serine Non-P.H.S. U.S. Gov't Non-U.S. Gov't Research Support Enzymology Pseudomonadaceae Protein Conformation Site-Directed mutagenesis Molecular Sequence Data kinetics cysteine circular dichroism binding sites Amino Acid Sequence acetylation metabolism genetics BIOSYNTHESIS Acetyl-CoA C-Acetyltransferase on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Adding L-3-(2-Naphthyl)alanine to the genetic code of E. coli
Lei Wang, Ansgar Brock, and Peter G Schultz
Journal of the American Chemical Society 124 (9), 1836-7 (06 Mar 2002)
info:pmid/11866580
Posted by gjwilliams2 to Tyrosine-tRNA Ligase Matrix-Assisted Laser Desorption-Ionization mass spectrometry P.H.S. U.S. Gov't Research Support Protein Conformation Site-Directed mutagenesis Molecular Sequence Data Escherichia coli bacterial DNA binding sites Amino Acid Sequence metabolism genetics chemistry analogs & derivatives Alanine on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Characterization of the surfactin synthetase C-terminal thioesterase domain as a cyclic depsipeptide synthase
Claire C Tseng et al.
Biochemistry 41 (45), 13350-9 (12 Nov 2002)
info:pmid/12416979
Posted by gjwilliams2 to beta-Alanine Thiolester Hydrolases Substrate Specificity P.H.S. U.S. Gov't Non-U.S. Gov't Research Support Tertiary protein structure chemical synthesis Cyclic Peptides Peptide Synthases antagonists & inhibitors Peptide Fragments Site-Directed mutagenesis macrolides Hydrolysis Enzyme Inhibitors analogs & derivatives cysteamine X-ray crystallography Boronic Acids Bacterial Proteins genetics Enzymology Bacillus subtilis chemistry Anti-Bacterial Agents Amino Acid Sequence on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution
G Saab-Rincón et al.
Protein engineering 14 (3), 149-55 (Mar 2001)
info:pmid/11342710
Posted by gjwilliams2 to Variation (Genetics) growth & development Enzymology Trypanosoma brucei brucei Triose-Phosphate Isomerase thermodynamics structure-activity relationship DNA Sequence analysis Sequence Alignment Non-U.S. Gov't Research Support Protein engineering polymerase chain reaction oligonucleotides Site-Directed Insertional mutagenesis molecular structure Molecular Models kinetics growth Gene Library Escherichia coli Enzyme Activation Directed Molecular Evolution metabolism DNA Ligases Catalysis binding sites animals genetics chemistry amino acids Amino Acid Substitution Amino Acid Sequence on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Mutagenesis of vitamin K-dependent carboxylase demonstrates a carboxyl terminus-mediated interaction with vitamin K hydroquinone
D A Roth et al.
The Journal of biological chemistry 270 (10), 5305-11 (10 Mar 1995)
info:pmid/7890642
Posted by gjwilliams2 to analogs & derivatives Vitamin K 1 transfection Substrate Specificity Spodoptera Sequence Deletion P.H.S. U.S. Gov't Research Support Recombinant Proteins Oligodeoxyribonucleotides Site-Directed Insertional mutagenesis Molecular Sequence Data Enzymology liver metabolism isolation & purification chemistry Ligases kinetics COMPARATIVE STUDY Codon Cell Line Cattle Carbon-Carbon Ligases Western blotting binding sites Base Sequence Baculoviridae animals Amino Acid Sequence on Thu Jul 03 2008 at 00:50 UTC | info | related

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