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Suicide substrates, mechanism-based enzyme inactivators: recent developments
C T Walsh
Annual review of biochemistry 53, 493-535 (1984)
info:pmid/6433782 | info:doi/10.1146/annurev.bi.53.070184.002425
Posted by gjwilliams2 to beta-Lactamases Substrate Specificity Serine Endopeptidases Pyrimidines metabolism Pyridoxal Phosphate protease inhibitors Oxidoreductases liver Lipoxygenase Inhibitors Isoenzymes Hydrolases Glycoside Hydrolases fluorine Enzyme Inhibitors Enzymology Endoplasmic Reticulum Dopamine beta-Hydroxylase DNA repair antagonists & inhibitors Cytochrome P-450 Enzyme System Cyclooxygenase Inhibitors chemistry Arachidonate Lipoxygenases animals Adenosylhomocysteinase on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase
F Rusnak et al.
Biochemistry 29 (6), 1425-35 (13 Feb 1990)
info:pmid/2139796
Posted by gjwilliams2 to Substrate Specificity analogs & derivatives Serine Research Support, U.S. Gov't, P.H.S. Research Support, Non-U.S. Gov't Promoter Regions (Genetics) Plasmids molecular structure Molecular Sequence Data Magnetic Resonance Spectroscopy kinetics isolation & purification Hydrolases Hydrogen-Ion Concentration Gene Expression Regulation, Enzymologic genetics Enzymology Escherichia coli metabolism enterobactin Cloning, Molecular Base Sequence on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Shared promiscuous activities and evolutionary features in various members of the amidohydrolase superfamily
Cintia Roodveldt and Dan S Tawfik
Biochemistry 44 (38), 12728-36 (27 Sep 2005)
info:pmid/16171387 | info:doi/10.1021/bi051021e
Posted by gjwilliams2 to Substrate Specificity Non-U.S. Gov't Research Support Phosphoric Triester Hydrolases mutation Hydrolysis Hydrolases Molecular evolution Esterases Escherichia coli Proteins Directed Molecular Evolution Dihydroorotase metabolism classification chemistry Amidohydrolases on Thu Jul 03 2008 at 00:50 UTC | info | related
 
Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes
S Raillard et al.
Chemistry & biology 8 (9), 891-8 (Sep 2001)
info:pmid/11564557
Posted by gjwilliams2 to Triazines Substrate Specificity structure-activity relationship Research Support, U.S. Gov't, P.H.S. proteins Mutagenesis, Site-Directed metabolism Hydrolases genetics chemistry Escherichia coli Directed Molecular Evolution Aminohydrolases on Thu Jul 03 2008 at 00:15 UTC | info | related
 
Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox
Mariël G Pikkemaat and Dick B Janssen
Nucleic acids research 30 (8), E35-5 (15 Apr 2002)
info:pmid/11937643
Posted by gjwilliams2 to Enzymology Xanthobacter Sequence Alignment Research Support, Non-U.S. Gov't mutation mutagenesis Molecular Sequence Data metabolism genetics Hydrolases Gene Library methods Directed Molecular Evolution Amino Acid Sequence on Thu Jul 03 2008 at 00:15 UTC | info | related
 
The behavior and significance of slow-binding enzyme inhibitors
J F Morrison and C T Walsh
Advances in enzymology and related areas of molecular biology 61, 201-301 (1988)
info:pmid/3281418
Posted by gjwilliams2 to protease inhibitors Phosphopyruvate Hydratase kinetics antagonists & inhibitors Hydrolases Pharmacology metabolism Enzyme Inhibitors Decarboxylation on Thu Jul 03 2008 at 00:15 UTC | info | related
 
Directed evolution and characterization of a novel D-pantonohydrolase from Fusarium moniliforme
Zhiqiang Liu, Zhihao Sun, and Yong Leng
Journal of agricultural and food chemistry 54 (16), 5823-30 (09 Aug 2006)
info:pmid/16881683 | info:doi/10.1021/jf060794m
Posted by gjwilliams2 and 1 other to transfection Substrate Specificity Stereoisomerism Research Support, Non-U.S. Gov't polymerase chain reaction Hydroxybutyrates Hydrolases Hydrogen-Ion Concentration gene expression Enzymology fusarium genetics Escherichia coli Enzyme Stability Directed Molecular Evolution DNA shuffling metabolism chemistry analogs & derivatives 4-Butyrolactone on Thu Jul 03 2008 at 00:15 UTC | info | related
 
Esterolytic antibodies as mechanistic and structural models of hydrolases-a quantitative analysis
Ariel B Lindner et al.
Journal of molecular biology 320 (3), 559-72 (12 Jul 2002)
info:pmid/12096909
Posted by gjwilliams2 to thermodynamics Substrate Specificity Research Support, Non-U.S. Gov't Protein Conformation Pharmacology Peroxides Mutagenesis, Site-Directed molecular structure Models, Molecular kinetics In Vitro Hydrolases Hydrogen-Ion Concentration hydrogen bonding Enzyme Stability COMPARATIVE STUDY Catalysis Antigens metabolism genetics chemistry antibodies animals on Thu Jul 03 2008 at 00:15 UTC | info | related
 
In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes
Hening Lin et al.
Journal of the American Chemical Society 127 (31), 11075-84 (10 Aug 2005)
info:pmid/16076215 | info:doi/10.1021/ja0522027
Posted by gjwilliams2 to Salmonella Research Support, U.S. Gov't, P.H.S. Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural kinetics Hydrolysis isolation & purification chemistry Hydrolases genetics Enzymology Escherichia coli Electrophoresis, Polyacrylamide Gel Cloning, Molecular Catalysis on Thu Jul 03 2008 at 00:15 UTC | info | related
 
Engineering cyclodextrin glycosyltransferase into a starch hydrolase with a high exo-specificity
Hans Leemhuis et al.
Journal of biotechnology 103 (3), 203-12 (15 Aug 2003)
info:pmid/12890607
Posted by gjwilliams2 to alpha-Amylase Substrate Specificity structure-activity relationship starch Research Support, Non-U.S. Gov't Recombinant Proteins methods Protein engineering Protein Conformation Protein Binding Mutagenesis, Site-Directed Models, Molecular Hydrolases Glucosyltransferases Enzyme Stability Enzyme Activation COMPARATIVE STUDY binding sites genetics Enzymology chemistry Bacillus stearothermophilus on Thu Jul 03 2008 at 00:15 UTC | info | related

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