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Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147
Z Wu, G D Wright, and C T Walsh
Biochemistry 34 (8), 2455-63 (28 Feb 1995)
info:pmid/7873524
Posted by gjwilliams2 to Pharmacology Vancomycin Substrate Specificity Serine-Type D-Ala-D-Ala Carboxypeptidase P.H.S. Non-P.H.S. U.S. Gov't Research Support chemistry Oligopeptides Molecular Sequence Data kinetics Hydrolysis Hydrogen-Ion Concentration Genes Escherichia coli Enzymology drug effects Enterococcus faecium microbial Drug resistance Dipeptidases bacterial DNA DNA Primers Molecular cloning competitive binding Base Sequence metabolism isolation & purification genetics Bacterial Proteins Amino Acid Sequence on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Phosphinate analogs of D-, D-dipeptides: slow-binding inhibition and proteolysis protection of VanX, a D-, D-dipeptidase required for vancomycin resistance in Enterococcus faecium
Z Wu and C T Walsh
Proceedings of the National Academy of Sciences of the United States of America 92 (25), 11603-7 (05 Dec 1995)
info:pmid/8524812
Posted by gjwilliams2 to Vancomycin Titrimetry Stereoisomerism Serine-Type D-Ala-D-Ala Carboxypeptidase Research Support, U.S. Gov't, P.H.S. Recombinant Proteins chemistry protease inhibitors Propionic Acids Pharmacology Organophosphorus Compounds kinetics drug effects Enterococcus faecium Drug Resistance, Microbial Dithionitrobenzoic Acid Dipeptides antagonists & inhibitors Dipeptidases COMPARATIVE STUDY binding sites metabolism genetics Bacterial Proteins on Thu Jul 03 2008 at 01:10 UTC | info | related
 
Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX
D G McCafferty, I A Lessard, and C T Walsh
Biochemistry 36 (34), 10498-10505 (26 Aug 1997)
info:pmid/9265630 | info:doi/10.1021/bi970543u
Posted by gjwilliams2 to zinc Thrombin thermolysin Serine-Type D-Ala-D-Ala Carboxypeptidase Sequence Alignment P.H.S. U.S. Gov't Non-U.S. Gov't Research Support isolation & purification Recombinant Fusion Proteins Protein Conformation Site-Directed mutagenesis Monosaccharide Transport Proteins Molecular Sequence Data chemical Models kinetics Escherichia coli Proteins Escherichia coli Enzymology Enterococcus Dipeptidases DNA Mutational Analysis Conserved Sequence circular dichroism Carrier Proteins binding sites metabolism genetics chemistry Bacterial Proteins Amino Acid Sequence ATP-Binding Cassette Transporters on Thu Jul 03 2008 at 00:15 UTC | info | related
 
Mutational analysis of active-site residues of the enterococcal D-ala-D-Ala dipeptidase VanX and comparison with Escherichia coli D-ala-D-Ala ligase and D-ala-D-Ala carboxypeptidase VanY
I A Lessard and C T Walsh
Chemistry & biology 6 (3), 177-87 (Mar 1999)
info:pmid/10074467 | info:doi/10.1016/S1074-5521(99)89009-7
Posted by gjwilliams2 to Vancomycin Serine-Type D-Ala-D-Ala Carboxypeptidase Research Support, U.S. Gov't, P.H.S. Research Support, Non-U.S. Gov't Protein Structure, Tertiary Peptide Synthases mutation Mutagenesis, Site-Directed membrane proteins Escherichia coli Enzymology Enterococcus Electrophoresis, Polyacrylamide Gel Drug Resistance, Microbial Dipeptidases DNA, Recombinant DNA Mutational Analysis COMPARATIVE STUDY Carboxypeptidases genetics BIOSYNTHESIS Bacterial Proteins Pharmacology Anti-Bacterial Agents on Thu Jul 03 2008 at 00:15 UTC | info | related
 
VanX, a bacterial D-alanyl-D-alanine dipeptidase: resistance, immunity, or survival function?
I A Lessard and C T Walsh
Proceedings of the National Academy of Sciences of the United States of America 96 (20), 11028-32 (28 Sep 1999)
info:pmid/10500118
Posted by gjwilliams2 to Pharmacology BIOSYNTHESIS Vancomycin Serine-Type D-Ala-D-Ala Carboxypeptidase Research Support, U.S. Gov't, P.H.S. Research Support, Non-U.S. Gov't Escherichia coli Drug Resistance, Microbial Dipeptidases Cell Survival physiology Bacterial Proteins immunology Enzymology drug effects bacteria on Thu Jul 03 2008 at 00:15 UTC | info | related
 
Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function
I A Lessard et al.
Chemistry & biology 5 (9), 489-504 (Sep 1998)
info:pmid/9751644
Posted by gjwilliams2 to zinc Pharmacology Vancomycin Substrate Specificity Streptomyces Stereoisomerism Serine-Type D-Ala-D-Ala Carboxypeptidase Sequence Alignment Research Support, Non-U.S. Gov't Protein Conformation Molecular Sequence Data Models, Molecular Escherichia coli Drug Resistance, Microbial Dipeptides Dipeptidases Enzymology Cyanobacteria cell wall Catalysis Base Sequence metabolism chemistry Bacterial Proteins Amino Acid Sequence on Thu Jul 03 2008 at 00:15 UTC | info | related
 
Directed evolution of alpha-aspartyl dipeptidase from Salmonella typhimurium
X Kong et al.
Biochemical and biophysical research communications 289 (1), 137-42 (23 Nov 2001)
info:pmid/11708790 | info:doi/10.1006/bbrc.2001.5937
Posted by gjwilliams2 to Enzymology Salmonella typhimurium Protein Conformation polymerase chain reaction Models, Molecular kinetics Hydrogen-Ion Concentration Genes, Bacterial Enzyme Stability Directed Molecular Evolution metabolism chemistry Dipeptidases DNA, Bacterial DNA Primers COMPARATIVE STUDY genetics Catalytic Domain Base Sequence Amino Acid Substitution on Thu Jul 03 2008 at 00:15 UTC | info | related

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