TipDCs (TNF and iNOS producing DCs) is an arbitrary and confusing name for these cells. Are they really dendritic cells? They don't express MHCII or CD11c! Of course the authors prove they transform into CD11c+ MHCII+ cells in-vitro upon stimulation, but so do monocytes, and monocytes are NOT dendritic cells. These cells appear to be of the mononuclear lineage (moncytes or something). Maybe, the many fancy names given to monocytes are just a reflection of the different properties of the same cell population (Gr1+ inflammatory monocytes) in different situations.
The way they identify these cells by flow cytometry is also confusing. Instead of using Ly6G to gate out neutrophils or using F480 to include only monocytes, they decided to gate on Ly6Chigh cells or Gr-1 high cells or even focus on all CD11b+ cells!
Of interest, their data shows that Gr-1+ monocytes from mice infected with L. monocytogenes transform into CD11c+ MHCII+ iNOS+ dendritic cells upon in-vitro stimulation with bacteria and IFN-g. Is this the same thing that happens to GR-1+ monocytes from tumor bearing mice when they start producing iNOS upon IFN-g stimulation, thereby inhibiting T-cell proliferation "myeloid suppressor cell" effect? Is cancer a chronic inflammatory stimuli which is going to generate/expand the same kind of iNOS producing Gr1+ monocytes seen during Listeriosis and other infections?
Also, nice data with flow cytometry for iNOS with Santa Cruz antibody.
The way they identify these cells by flow cytometry is also confusing. Instead of using Ly6G to gate out neutrophils or using F480 to include only monocytes, they decided to gate on Ly6Chigh cells or Gr-1 high cells or even focus on all CD11b+ cells!
Of interest, their data shows that Gr-1+ monocytes from mice infected with L. monocytogenes transform into CD11c+ MHCII+ iNOS+ dendritic cells upon in-vitro stimulation with bacteria and IFN-g. Is this the same thing that happens to GR-1+ monocytes from tumor bearing mice when they start producing iNOS upon IFN-g stimulation, thereby inhibiting T-cell proliferation "myeloid suppressor cell" effect? Is cancer a chronic inflammatory stimuli which is going to generate/expand the same kind of iNOS producing Gr1+ monocytes seen during Listeriosis and other infections?
Also, nice data with flow cytometry for iNOS with Santa Cruz antibody.